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MilliporeSigma

ROCHE Pvu II, from Proteus vulgaris

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Storage: −20°C

UNSPSC Code: 12352200

Components: Enzyme Solution; SuRE/Cut Buffer M 10x concentrated

RIDADR: NONH for all modes of transport

Analysis Note:
SuRE/Cut Buffer System
The buffer in bold is recommended for optimal activity
• A: 25-50%
• B: 25-50%
• H: 25-50%
• L: 25-50%
• M: 100%

General description:
Pvu II recognizes the sequence CAG↓*CTG and generates fragments with blunt ends.

Other Notes:
For life science research only. Not for use in diagnostic procedures.

Quality:
Absence of nonspecific endonuclease activities
1μg λDNA is incubated for 16 hours in 50μl SuRE/Cut Buffer M with an excess of Pvu II. The number of enzyme units which do not change the enzyme-specific pattern is stated in the certificate of analysis.

Absence of exonuclease activity
Approximately 5μg [3H] labeled calf thymus DNA are incubated with 3μl Pvu II for 4 hours at +37°C in a total volume of 100μl 50mM Tris-HCl, 10mM MgCl2, 1mM Dithioerythritol, pH approximately 7.5. Under these conditions, no release of radioactivity is detectable, as stated in the certificate of analysis.

Specifications:
Compatible ends
The enzyme generates ends that are compatible with any other blunt end.

Isoschizomers
The enzyme is not known to have isoschizomers.

Methylation sensitivity
Pvu II is inhibited by 5′- and 4′-methylcytosine at the site indicated (*) in the sequence CAG*CTG.

Incubation temperature
+37°C

DNA Profile:
Number of cleavage sites on different DNAs
• λ: 15
• φX174: 0
• Ad2: 24
• M13mp7: 3
• pBR322: 1
• pBR328: 1
• pUC18: 2
• SV40: 3

Specificity:
Star Activity
Pvu II exhibits star activity under non-optimal conditions. The relaxed sequence specificity of Pvu II includes all sequence combinations which differ by one nucleotide from the non-relaxed sequence (e.g., CCGCTG, CATCTG, CAGATG).
Recognition sites: CAG*CTG
CAG*CTG
Restriction site: CAG↓*CTG
CAG↓*CTG
Heat inactivation: No inactivation of Pvu II after incubation at 65 °C for 15 minutes.

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Product Detail
Thomas No.
C756X11
Mfr. No.
10642690001
Description
Pvu II, 1000 U

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$0.00
Thomas No.
C756X12
Mfr. No.
10642703001
Description
Pvu II, 5000 U

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