Poly-L-lysine solution mol wt 70,000-150,000, 0.01%, sterile-filtered, BioReagent, suitable for cell culture

Synonym: PDL Solution

CAS Number: 25988-63-0

Empirical Formula (Hill Notation): C₁₆H₁₅N₅ · 2HCl

MDL Number: MFCD00165424

Application
Recommended as a cell culture substratum when using 0.5 mL of a 0.1 mg/ml solution to coat 25 cm². Optimal conditions for attachment must be determined for each cell line and application.

Other Notes
This poly-L-lysine is relatively low density which provides good handling characteristics. However, it may be degraded by some cell types. In that case a poly-D-lysine should be chosen.

General Description
Poly-lysine polymers are useful molecules to prepare surfaces for cell attachment. Poly-lysine polymers are available in L and D conformations. Poly-L-lysine polymers can be digested by some cell types, whereas poly-D-lysines are stable. Poly-lysine polymers from 70 to 300 kDa provide sufficient attachment site density for good cell adherence. However, higher the molecular weight poly-lysine polymers are more viscous and difficult to work with.

The choice of poly-lysine polymer for a specific cell type and application should account for the susceptibility of the polymer to digestion, the required attachment site density and the difficulty in handling.