Isopropanol is a clear, colorless, polar organic solvent commonly used in chemistry and molecular biology laboratories. It will dissolve a wide range of chemicals, and evaporates quickly. This product (2-propanol) is designated as molecular biology grade and is suitable for the precipitation of nucleic acids.Protocols for the use of isopropanol:
•DNA precipitation from mammalian tissue, ethidium bromide extraction from DNA samples, and RNA isolation from cells and tissues.
•Protein crystallization.
•Isolate retinoids from biological matrices for HPLC and HPLC-MS analysis.
Synonyms: sec-Propyl alcohol; IPA; Isopropanol; Isopropyl alcohol
Molecular Formula: C3H8O
Molecular Weight: 60.1
Linear Structural Formula: (CH3)2CHOH
MDL Number: MFCD00011674
Purity: >=99.5%
Melting Point: -89.5 C (lit.)
Density: 0.785 g/mL at 25 C (lit.)
Refractive Index: n20/D 1.377 (lit.)
Storage: room temp
Application: 2-Propanol has been used:
•in DNA and RNA extraction
•for determination of hydroxyproline levels(The Merck Index, 12th ed)
•for checking cell viability by MTT assay
•in microarray hybridizationSuitable for use in DNA precipitation using standard protocols. Also suitable as a solvent for making solutions for molecular biology applications.
Grade: for molecular biology
Vapor density: 2.1 (vs air)
Vapor pressure: 33 mmHg ( 20 °C); 44 mmHg ( 25 °C)
Product line: BioReagent
Assay: =99.5%
Form: liquid
Autoignition temp.: 750 °F
Expl. lim.: 2 %
Refractive index: n20/D 1.377(lit.)
BP: 82 °C(lit.)
MP: -89.5 °C(lit.)
Density: 0.785 g/mL at 25 °C(lit.)
Suitability: suitable for precipitation of nucleic acids
Storage temp.: room temp