The OxyIHC Oxidative Stress Detection Kit (S7450) contains the chemical and immunological reagentsnecessary to detect protein oxidation in various tissues from a variety of organs and animal species. TheOxyIHC test method involves chemical derivatization of protein carbonyl groups with 2,4-dinitrophenylhydrazine (DNPH). This chemical reaction results in proteins being covalently coupled to DNPat their carbonyl sites. The DNP-derivatized proteins are then detected using an antibody that specificallybinds to the DNP moiety. Subsequent incubation with biotin conjugated secondary antibody, streptavidinconjugated HRP, and development using a 3,3' diaminobenzidine (DAB) staining allowsimmunohistochemical detection of protein oxidation.
Application: The OxyIHC Oxidative Stress Detection Kit contains the chemical & immunological reagents necessary to detect protein oxidation in various tissues from a variety of organs & animal species.