Synonyms: β-Nicotinamide adenine dinucleotide phosphate disodium salt; β-NADH phosphate disodium salt; β-NADP-Na2; Triphosphopyridine nucleotide disodium salt
Empirical Formula (Hill Notation): C21H26N7Na2O17P3
Formula Weight: 787.37
Linear Formula: C21H26N7Na2O17P3
MDL Number: MFCD00065390
Purity: ‚≥97%
Storage: 2-8°C
UNSPSC Code: 12352200
RIDADR: NONH for all modes of transport
Analysis Note:
Absorbance coefficients: 18.0 x 103 [l x mol-1 x cm-1] at 260 nm, 5.9 x 103 [l x mol-1 x cm-1] at 333 nm (CN complex)
Absorbance at 340 nm: Imidazol buffer, 100 mM pH 6.7, NADP 2 mM (= 14.87 mg/10 ml) : A340 approximately 0.100.
Absorbance at 360 nm: Aqueous solution of NADP, 10 mg/ml: A360 approximately 0.200-0.250.
Application:
The β-NADH phosphate disodium salt has been used for the quantification of glucose in glycogen. It has also been used for the hexokinase assay.
General description:
Nicotinamide adenine dinucleotide phosphate (NADP) and NADPH form a redox pair. The NADPH/NADP ratio regulates the intracellular redox potential, thereby influencing metabolic reactions in vivo.
Other Notes:
For life science research only. Not for use in diagnostic procedures.
Preparation Note:
Storage conditions (working solution): 2 to 8 °C
10 mg/ml in aqueous solution, contains at least 99% NADP after 4 weeks at 2 to 8 °C