Synonyms: Alpha Smooth Muscle Actin Antibody - Monoclonal Anti-Actin, alpha-Smooth Muscle; Alpha Smooth Muscle Actin Antibody Sigma; Anti-Alpha Smooth Muscle Actin Antibody; SMA
Storage: -20C
UNSPSC Code: 12352203
General description: The antibody (also known as anti-alpha-Sm-1) is specific for the single isoform of alpha-smooth muscle actin. It reacts specifically with alpha-smooth muscle actin in immunoblotting assays and labels smooth muscle cells in frozen or formalin-fixed, paraffin-embedded tissue sections.
General description: Actins are cytoskeletal proteins that regulate cell motility. Several scaffolding, signaling and actin-binding proteins regulate the interconversions between filamentous F-actins and monomeric G-actins. Cellular actins of various species have very similar immunological and physical properties. Hence, the use of monoclonal antibody to actin provides a specific and useful tool for studying the localization, structure and function of actin proteins.Mouse monoclonal anti-actin, alpha-smooth muscle antibody binds to actin from human, mouse, rat, bovine, chicken, frog, goat, guinea pig, rabbit, dog, sheep, and snake.
Application: Immunocytochemistry was performed on smooth muscle cells from bovine aortas using the monoclonal anti-ACTA2 antibody. Cells were first grown on glass cover slips and fixed in 50% acetone/EtOH for 10 minutes at 4 degrees.
Application: Mouse monoclonal anti-actin, alpha-smooth muscle antibody has been used for western blot assays using whole kidney homogenates. The product has also been used for immunohistochemical assays using mice tissue sections.
Application: Paraffin embedded sections of rat testis tissue grafts were immunohistochemically stained with mouse monoclonal anti-smooth muscle actin. IHC analysis of x-gal stained muouse cardiac tissue was performed using the primary antibody, mouse monoclonal anti-smooth muscle actin to identify myofibroblasts.
Immunogen: N-terminal synthetic decapeptide of alpha-smooth muscle actin.
Other Notes: To view an Actin antibody selection guide, please visit www.sigmaaldrich.com/actin.
RIDADR: NONH for all modes of transport
WGK Germany: 3