With a similarity to humans in heart size, blood flow rate, skin, liver enzymes and bone healing, porcine models have replaced many canine and non-human primate models in many research areas such as cardiovascular, toxicology, pharmacology, aging and bone. "Minipigs," bred to retain the similar heart size and function as humans, but age without growing too large, have been used for long term preclinical or multi-generational studies which may be performed in a comparatively short period of time. MILLIPLEX(R) Porcine Cytokine/Chemokine Panel can be used for the simultaneous quantification of any or all of the following analytes in porcine tissue/cell lysate and culture supernatant samples and serum or plasma samples: GM-CSF, IFNgamma, IL-1alpha, IL-1ra, IL-1beta, IL-2, IL-4, IL-6, IL-8, IL-10, IL-12, IL-18 and TNFalpha. This kit uses a 96-well format, contains a lyophilized standard cocktail, two internal assay quality controls and can measure up to 38 samples in duplicate. The Luminex(R) xMAP(R) platform uses a magnetic bead immunoassay format for ideal speed and sensitivity to quantitate multiple analytes simultaneously, dramatically improving productivity while conserving valuable sample volume. Panel Type: Cytokines/Chemokines
Application:
•Analytes: GM-CSF, IFNgamma, IL-1alpha, IL-1beta, IL-1Ra, IL-2, IL-4, IL-6, IL-8 (CXCL8), IL-10, IL-12, IL-18, TNF-alpha
•Recommended Sample Type: serum, plasma or cell/tissue culture supernatants or lysate
•Recommended Sample Dilution: 25 muL per well of undiluted serum or plasma; cell/tissue culture samples may require dilution in an appropriate control medium.
•NOTE: Extra centrifugation and attention may be needed for samples with extremely high lipid content.
•Assay Run Time: Overnight (16-18 hours) at 2-8C.
•Research Category: Inflammation & Immunology
Legal Information: xMAP is a registered trademark of Luminex Corp
Other Notes: Sensitivity: Refer to kit protocol for sensitivities of individual biomarkers.