Angiogenesis, the development of new vascular networks, is a key process in normal growth and development as well as in wound healing, with homeostasis maintained by a delicate balance of angiogenic factors and inhibitors. Consequently, insufficient, or excessive blood vessel growth underlies many diseases, including cardiovascular disease, diabetic ulcers, macular degeneration, and cancer. Angiogenesis plays a significant role in tumor growth and metastasis. MILLIPLEX(R) Human Angiogenesis Magnetic Bead Panel 2 is a 20-plex kit to be used for the simultaneous quantification of any or all of the following analytes in serum, plasma, cell culture samples, and tissue homogenates/lysates: Angiostatin, soluble E-Selectin, (sE-Selectin), Osteopontin (OPN), Platelet Derived Growth Factor-AB/BB (PDGF-AB/BB), soluble Platelet Endothelial Cell Adhesion Molecule-1 (sPECAM-1), Tenascin C, Thrombospondin-2 (TSP-2), soluble AXL (sAXL), soluble c-Kit/Stem Cell Growth Factor Receptor (sc-Kit/SCFR), soluble Epidermal Growth Factor Receptor (sEGFR)†, soluble Human Epidermal Growth Factor Receptor 2 (sHer2), soluble Human Epidermal Growth Factor Receptor 3 (sHer3), soluble Hepatocyte Growth Factor Receptor/c-Met (sHGFR/c-Met), soluble IL-6Ralpha, soluble Neuropilin-1 (sNRP-1), soluble Tie-2 (sTie-2), soluble Urokinase-type Plasminogen Activator Receptor (suPAR), soluble Vascular Endothelial Growth Factor Receptor 1 (sVEGFR1), soluble Vascular Endothelial Growth Factor Receptor 2 (sVEGFR2), and soluble Vascular Endothelial Growth Factor Receptor 3 (sVEGFR3). This kit uses a 96-well format, contains a lyophilized standard cocktail, two internal assay quality controls and can measure up to 38 samples in duplicate. †Antibody against active site results in preferential binding to unbound EGFR. The Luminex(R) xMAP(R) platform uses a magnetic bead immunoassay format for ideal speed and sensitivity to quantitate multiple analytes simultaneously, dramatically improving productivity while conserving valuable sample volume. Panel Type: Circulating Cancer
Application:
•Analytes: Angiostatin, soluble AXL (sAXL) , soluble c-Kit/Stem Cell Growth Factor Receptor (sc-Kit/SCFR), soluble E-Selectin (sE-Selectin), soluble Epidermal Growth Factor Receptor (sEGFR/sErbB1/sHER1)†, soluble Human Epidermal Growth Factor Receptor 2 (sHer2/sHER2/sErbB2), soluble Human Epidermal Growth Factor Receptor 3 (sHer3/ sHER3 / sErbB3), soluble Hepatocyte Growth Factor Receptor (sHGFR/c-Met/sc-Met), soluble IL-6Ralpha (IL-6Ralpha (soluble)/sIL-6Ralpha), soluble Neuropilin-1 (sNRP-1), Osteopontin (OPN), Platelet Derived Growth Factor-AB/BB (PDGF-AB/BB), soluble Platelet Endothelial Cell Adhesion Molecule-1 (sPECAM-1/sCD31), Tenascin C (TN-C), Thrombospondin-2 (TSP-2), soluble Tie-2 (sTie-2), soluble Urokinase-type Plasminogen Activator Receptor (suPAR), soluble Vascular Endothelial Growth Factor Receptor 1 (soluble VEGFR1, sVEGFR1/ sFlt-1), soluble Vascular Endothelial Growth Factor Receptor 2 (soluble VEGFR2/sVEGFR2/ sKDR/sFlk-1), soluble Vascular Endothelial Growth Factor Receptor 3 (soluble VEGFR3/sVEGFR3/ sFlt-4).
•†Antibody against active site results in preferential binding to unbound EGFR.
•Recommended Sample Type: Human serum, plasma, cell culture supernatants and tissue/cell homogenates/lysates
•Recommended Sample Dilution: 25 muL per well of 1:5 diluted plasma or serum; cell culture supernatant may be used undiluted or diluted with appropriate control medium
•Assay Run Time: Overnight (16-18 hours) at 2-8C
•Research Category: Cancer
Legal Information: xMAP is a registered trademark of Luminex Corp
Other Notes: Sensitivity: See kit protocol for individual analytes sensitivities.