CAS Number: 37340-57-1
MDL Number: MFCD00131560
Lyticase hydrolyzes poly-β(1→3)-glucose such as yeast cell wall glucan.
One unit will produce a ΔA800 of 0.001 per min at pH 7.5 at 25 °C, using a suspension of yeast as substrate in a 3 mL reaction mixture.
Partially purified, lyophilized powder containing potassium phosphate buffer salts and stabilizers.
Yeast cells are difficult to disrupt because the cell walls may form capsules or resistant spores. DNA can be extracted from yeast by using lysing enzymes such as lyticase, chitinase, zymolase, and gluculase to induce partial spheroplast formation; spheroplasts are subsequently lysed to release DNA. Lyticase is preferred to digest cell walls of yeast and generate spheroplasts from fungi for transformation.
Reported to be useful for lysis of Ashbya, Candida, Debaryomyces, Eremothecium, Endomyces, Hansenula, Hanseniaspora, Kloeckera, Kluyveromyces, Lipomyces, Metschikowia, Pichia, Pullularia, Torulopsis, Saccharomyces, Saccharomycopsis, Saccharomycodes, and Schwanniomyces species.
Quality Level: PREMIUM
Form: lyophilized powder
Composition: Protein, ≥20% biuret
Storage Temp.: −20°C