The Live-Dead Cell Viability Assay Kit is a quick and simple three-color assay to measure cell viability. The kit consists of Calcein-AM (stains live cells), Propidium Iodide (stains dead cells) and Hoechst 33342 (stains all cells). The kit has been optimized for 3D cell culture (spheroids, human organoids and 3D matrices) and 2D cell culture (multiple cell types). The kit can be used for flow cytometry, fluorescence microscopy and with fluorescence microplate readers. The kit determines viability of cells based on plasma membrane integrity and esterase activity. One kit contains reagents sufficient for 5 x 24-well plates or 12 x 96-well plates.Advantages: 1. Tested and optimized for 3D cell culture, Spheroid culture, organoid culture, 2D cell culture and flow cytometry.2. Easy to use and quick protocol.3. Stronger intensity of Calcein AM and Hoechst dyes compared to other products.Spectral PropertiesCalcein AM: Ex/Em: 490/515 nm (Green)Propidium Iodide: Ex/Em: 535/617 nm (Red)Hoechst 33342: Ex/Em: 361/486 nm (Blue)Protocols:Protocols for staining 2D monolayer cell culture, 3D cell culture, 3D spheroids and 3D organoids are included in the datasheet.