Synonym: Triacylglycerol acylhydrolase, Triacylglycerol lipase
CAS Number: 9001-62-1
EC Number: 232-619-9
Enzyme Commission (EC) Number: 3.1.1.3
MDL number: MFCD00131509
Biochem/physiol Actions
Tri-, di-, and monoglycerides are hydrolyzed (in decreasing order of rate).
Other Notes
Substrate specificity: preferentially hydrolyzes the 1- and 3-positions(middle to long fatty acids); optimum pH 5.0-7.0; optimum temperature 30-45°C; Selective enzymatic removal of protecting functions (C-terminal COOH function)1
Note: When triacetin is used as substrate, the pH is 7.4. Incubation time: 60 minutes.
Unit Definition
1 U corresponds to the amount of enzyme which liberates 1 μmol fatty acid from a triglyceride per minute at pH 7.7 and 40°C (olive oil as substrate)]; 300 U as described above are equivalent to ~1 U using triolein, Fluka No. 62314, at pH 8.0 and 40°C as substrate.