UNSPSC Code: 12352200
Cell Line Description: The KF1 cell line was developed from Koi carp (Cyprinus carpio koi) fin.
Cell Line Origin: Koi carp fin cell line, koi herpesvirus isolation
Culture Medium: EMEM (EBSS) + 2 mM Glutamine + 1% Non Essential Amino Acids (NEAA) + 10% Fetal Bovine Serum (FBS). If a CO2 incubator is not available, 10 mM HEPES can be included.
DNA Profile: Not specified
Subculture Routine: Split sub-confluent cultures (70-80%) 1:3 to 1:4, i.e., seeding at 2 x 104 cells/cm2 using 0.25% trypsin or trypsin/EDTA; 5% CO2; 25 C.
Other Notes: Cultures from HPA Culture Collections and supplied by Sigma are for research purposes only. Enquiries regarding the commercial use of a cell line are referred to the depositor of the cell line. Some cell lines have additional special release conditions such as the requirement for a material transfer agreement to be completed by the potential recipient prior to the supply of the cell line. Please view the Terms & Conditions of Supply for more information.
RIDADR: NONH for all modes of transport
WGK Germany: 3