JumpStart(TM) Taq ReadyMix(TM) for Quantitative PCR, For probe-based real-time PCR

Storage: -20C

UNSPSC Code: 41106300

Features and Benefits: . Ultimate Convenience. ReadyMixes contain all components necessary for QPCR, simply add fluorescent detection chemistry, primers, and template. Greater Specificity & Increased Target Yield. JumpStart Taq prevents non-specific amplification and increased target yield. Maximum Flexibility. Suitable for use with a variety of detection chemistries including molecular probes and double-stranded binding dyes such as SYBR Green I

Application: JumpStart(TM) Taq ReadyMix(TM) for Quantitative PCR has been used for qPCR amplifications using probes (during assay of HIV-1 RNA, DNA and inflammatory mRNAs). It has been used for Real-time PCR analysis of total extracted RNA in a study to monitor the activity of licorice in Treg cell differentiation and function.

Principle: The ReadyMix contains JumpStart Taq DNA Polymerase, 99% pure deoxynucleotides, and buffer in an optimized 2x concentrate. To prepare a reaction, 25 muL of the ReadyMix is added to primers, template, detection chemistry, and water for a total reaction volume of 50 muL. Set up can be performed at room temperature since the JumpStart Taq antibody renders the Taq DNA polymerase inactive. During the first denaturation cycle, the antibody dissociates from the enzyme and full activity is restored. No special preparations or protocol changes are required.

Unit Definition: One unit incorporates 10 nmol of total dNTPs into acid-precipitable DNA in 30 min. at 74 C.

Other Notes: JumpStart Taq ReadyMix for Quantitative PCR combines the advantages of a hot start enzyme with a ready-to-use mix for high throughput, quantitative PCR (qPCR). It is formulated without a detection chemistry making it suitable for use with a variety of formats including dual-labeled probes, molecular beacons, or double stranded binding dyes such as SYBR Green I.