MDL No.: MFCD00166026
Storage: -20C
UNSPSC Code: 12352204
Features and Benefits: . Reduces non-specific amplification. Increases PCR specificity and yield. Reduces set-up time concerns associated with manual or wax Hot Start methods. Activation time of less than 1 minute
Application: . For PCR amplifications that require reduced non-specific amplification. For multiplex PCR. For reduction of primer dimers
Application: JumpStart(R) Taq DNA Polymerase is used for real time reverse-transcription polymerase chain reaction analysis (Real-Time PCR) in the following ways: . Used for generation of complementary DNAs. Genomic DNA isolation. Isolation of total RNA from cultured cells. IL-4R and IL-13R analysis
Unit Definition: One unit incorporates 10 nmol of total dNTPs into acid-precipitable DNA in 30 min at 74 C.
Other Notes: View more detailed information on JumpStart Taq enzymes at www.sigma-aldrich.com/hotstart.
Other Notes: Sigma's JumpStart Taq DNA Polymerase is an antibody-inactivated hot-start enzyme designed to minimize non-specific amplification while increasing target yield. Once the reaction temperature reaches 70C, Taq DNA polymerase activity is restored and the resulting PCR exhibits a higher specificity and yield. This antibody-enzyme complex allows for easy and convenient set-up with less contamination risk than manual hot-start techniques. The enzyme may also be included in the master mix preparation resulting in more consistency from one reaction to the next.
RIDADR: NONH for all modes of transport