JumpStart(TM) AccuTaq(TM) LA DNA Polymerase, Hot-start high fidelity Taq enzyme, 10X buffer included

Synonyms: JumpStart(R) AccuTaq(TM) LA DNA Polymerase

Storage: -20C

UNSPSC Code: 12352200

Features and Benefits: . JumpStart AccuTaq LA DNA polymerase, an antibody inactivated hot start enzyme, is designed to minimize non-specific amplification while increasing target yield & specificity . Up to 6.5x greater fidelity in comparison to Taq DNA polymerase making it the enzyme of choice for multiplex PCR. Produce amplicons up to 22 kb with genomic templates and up to 40 kb with less complex templates such as lambda or bacterial genomic DNA. Reduce set-up time and eliminate concerns associated with manual or wax hot-start methods

Application: JumpStart AccuTaq LA DNA Polymerase is a combination of AccuTaq LA DNA Polymerase and a Taq-directed antibody. JumpStart Taq antibody reversibly binds to the AccuTaq LA DNA Polymerase, inactivating it at room temperature. The increased temperature of the first denaturation cycle causes the complex to dissociate, restoring the enzyme activity. This hot start mechanism provides increased specificity and higher target yield in comparison to standard amplification. JumpStart AccuTaq LA DNA Polymerase can generate long products with higher fidelity (up to 6.5x of Taq DNA polymerase).

Unit Definition: One unit incorporates 10 nmol of total dNTPs into acid-precipitable DNA in 30 min. at 74 C.

Other Notes: View more detailed information on Accutaq products at www.sigma-aldrich.com/hotstart.