Isopropyl β-D-1-thiogalactopyranoside, =99% (TLC)

Synonyms: IPTG; Isopropyl β-D-thiogalactoside

Formula: C₉H₁₈O₅S

Formula Weight: 238.30

CAS No.: 367-93-1

Purity: ≥99% (TLC)

Storage Temperature: 2-8°C

Application: IPTG is commonly used in cloning procedures that require induction of β-galactosidase activity. It is used in conjunction with X-Gal or Bluo-Gal in blue-white selection of recombinant bacterial colonies that induce expression of the lac operon in Escherichia coli. IPTG functions by binding to the lacI repressor and altering its conformation, which prevents the repression of the β-galactosidase coding gene lacZ.

Reconstitution: A stock solution (0.1 M) is prepared by dissolving IPTG in water with subsequent sterile filtration of the solution. The final concentration of IPTG in indicator plates should be 0.2 mM.

Supplemental Hazard Statements: May form explosive peroxides.

Other Notes: Non-metabolizable galactose analog.