Ready to use, cell culture-tested, suitable for stem cell applications. Acts as a highly potent, cell-permeable, reversible, and selective inhibitor of Rho-associated protein kinases (Ki = 140 nM for p160ROCK). Also shown to improve survival of human stem cells especially during cell culture passage and manipulation.
Synonyms: InSolution Y-27632, Sterile-Filtered, Cell Culture-Tested - CAS 146986-50-7 - Calbiochem
Molecular Formula: C14H21N3O · 2HCl
Molecular Weight: 320.26
Linear Structural Formula: C14H21N3O · 2HCl
Purity: >=95% (HPLC)
Application: InSolution Y-27632, Sterile-Filtered, Cell Culture-Tested, CAS 146986-50-7, is a 10 mM sterile-filtered solution of Y-27632 in H₂O. Acts as an inhibitor of Rho kinases (Ki = 140 nM for p160ROCK).
Biochem Physiol Actions: Primary TargetROCK
Legal Information: CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
Other Notes: Li X. et al, et. Al., 2009. Human Reproduction, 24, 580.Chitaley, K., et al. 2001. Nat. Med.7, 119.Davies, S.P., et al. 2000. Biochem. J.351, 95.Narumiya, S., et al. 2000. Methods Enzymol.325, 273.Hirose, M., et al. 1999. J. Cell Biol.141, 1625.Maekawa, M., et al. 1999. Science.285, 895.Uehata, M., et al. 1997. Nature.389, 990.