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Synonyms: 4-(2-Hydroxyethyl)piperazine-1-ethanesulfonic acid; N-(2-Hydroxyethyl)piperazine-N'-(2-ethanesulfonic acid)
Molecular Formula: C8H18N2O4S
Molecular Weight: 238.3
Linear Structural Formula: C8H18N2O4S
MDL Number: MFCD00006158
Application: HEPES is a biological buffer often referred to as a "Good's" buffer. It is the most commonly used biological buffer since the pKa of HEPES is 7.31 which corresponds to the physiological pH of 7.36. HEPES is considered to be non-toxic to culture cell lines, highly water soluble and provides high solution clarity.HEPES is used in cell culture media, in biopharma buffer formulations (both upstream and downstream), and in diagnostic reagents. HEPES based buffers are used in purification bioprocesses of antibodies, bioactives, proteins and growth factors.