UNSPSC Code: 12352200
RIDADR: NONH for all modes of transport
Features and Benefits:
• Isolate DNA-free miRNA ideal for qualitative and quantitative reverse transcription. ideal for qualitative and quantitative reverse transcription.
• Obtain excellent performance and linearity in RT-PCR.
• Generate stable , highly pure miRNAs using a simple, efficient protocol.
• Purify miRNA without using hazardous organic solvents. Avoid phenol/chloroform steps required by other suppliers′ miRNA purification kits.
• Choose one flexible kit for all your miRNA purifications. Use the same kit to purify small RNAs from a variety of sample types.
Application:
The High Pure miRNA Isolation kit rapidly purifies RNA that is suitable for direct use in many downstream applications:
• Northern blotting
• cDNA synthesis
• Primer extension
• miRNA array hybridization
• Relative quantification of miRNA with RT-PCR using, for example, the LightCycler® 480 System
General description:
In the presence of a chaotropic salt, RNA binds selectively to special glass fibers prepacked in the High Pure Spin Filter Tube. Bound RNA is purified in a series of rapid wash-and-spin steps to remove contaminating salts, proteins, and other cellular impurities, and then eluted using a low-salt solution. By lowering the concentration of Binding Enhancer during the binding step in the two-column protocol, the small RNA-containing miRNA passes the first column unbound. When the concentration of Binding Enhancer is increased, the small RNA fraction can be bound to a second High Pure Spin Filter Tube.
Other Notes:
Article (PDF, 176.3 KB)
Quality:
More than 60% recovery is obtained when 1 μg miRNA is added to106 K-562 cells and isolated with the one-column method, and more than 50% is recovered with the two-column method.
Components:
• Tissue Lysis Buffer (for FFPE sections)
• Proteinase K, recombinant (for FFPE sections)
• Binding Buffer
• Binding Enhancer
• Wash Buffer
• Elution Buffer
• High Pure Filter Tubes
• Collection Tubes
Legal Information:
LightCycler is a registered trademark of Roche