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MilliporeSigma

HiTrap® Heparin High Performance Cytiva, 17-0407-03

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HiTrap® Heparin HP columns are prepacked with Heparin Sepharose® High Performance for high-resolution purification of proteins with affnity for heparin such as DNA-binding proteins, coagulation factors, lipoproteins, and protein synthesis factors.Immobilized heparin has two main modes of interaction with proteins. It can operate as an affinity ligand with affinity for a wide range of biomolecules, including coagulation factors and other plasma proteins, lipoproteins, protein synthesis factors, enzymes that act on nucleic acids, and steroid receptors. Heparin also functions as a high capacity cation exchanger, due to its anionic sulfate groups. Gradient elution with salt is most commonly used in both cases.Heparin is coupled to the Sepharose® High Performance base matrix via the N-hydroxysuccinamide coupling method, to give high capacity, performance, and low leakage levels. HiTrap® Heparin HP is delivered in 0.2 M NaCl with 20% ethanol as bacteriostatic agent. Store HiTrap® Heparin HP columns equilibrated with 25 mL of 20% ethanol or a buffer containing a bacteriostatic agent. Equilibration is easily performed with a syringe and the supplied Luer adapter. Seal the column with the supplied stopper and domed nut. The recommended storage temperature is 4 C to 30 C.

Legal Information: Sepharose is a registered trademark of Cytiva

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Product Detail
Thomas No.
CHM01W348
Mfr. No.
GE17-0407-03
Description
GE17-0407-03 HiTrap(R) Heparin High Performance Cytiva, 17-0407-03, pack of 5 x 5 mL UN1170 - class 3 - PG 3 - Ethanol, solution
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Product is restricted and can only be purchased by customers with a web profile linked to a Thomas Business Account - Click here to login or create your web profile. If you do not have a Thomas Business Account, please Contact Us and someone will respond with details on how to apply for a business account with us.

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