Synonyms: Guanosine 5′-triphosphate sodium salt hydrate; 5′-GTP-Na2
Empirical Formula (Hill Notation): C10H16N5O14P3 ¬? xNa+ ¬? yH2O
Formula Weight: 523.18 (anhydrous free acid basis)
Linear Formula: C10H16N5O14P3 ¬? xNa+ ¬? yH2O
MDL Number: MFCD00149227
Purity: >90% (HPLC)
Beilstein Registry Number: 4113439
UNSPSC Code: 12352200
RIDADR: NONH for all modes of transport
Application:
GTP, disodium salt, is required as a coenzyme for protein biosynthesis in a cell-free system. It has been used in FtsZ polymerization assay and in the preparation of pipette solution for the electrophysiological recordings of the whole-cell membrane currents.
General description:
GTP functions as a carrier of phosphates and pyrophosphates involved in channeling chemical energy into specific biosynthetic pathways. It also serves as an energy-rich precursor of mononucleotide units in the enzymatic biosynthesis of DNA and RNA. GTP is involved in the initiation of peptide synthesis during the binding of formylmethionyl-tRNA (F-met-tRNA) to the ribosome. It is also involved in polypeptide chain elongation. GTP is hydrolyzed by tubulin and its hydrolysis is accompanied by microtubule assembly.
Other Notes:
For life science research only. Not for use in diagnostic procedures.
Quality:
Purity: 76% GTP (enzymatic), >90 area% (HPLC), 9% Na+, 8% H2O
Preparation Note:
Activator: EDTA prevents the formation of enzyme aggregates. Phosphate stimulates the NADH-dependent reaction.
Storage conditions (working solution): Aqueous solutions (pH 7.0) are stable at 2 to 8 °C or frozen for approx. one week, for reasons of hydrolysis pH should not be < 3.
If neutralized with water preparation can be stored frozen at -15 to -25 °C in small portions, one sample should thawed only once and should be used within the next one or two days (stored at 2 to 8 °C). Preparation can also be dissolved in a buffer pH 7-8 without neutralization (buffer concentration should be high enough that pH is not changed by addition of those substances); solution can be stored frozen.
Investigations on the storage temperature 2 to 8 °C compared to -15 to -25 °C showed no difference.