We are committed to bringing you Greener Alternative Products, which adhere to one or more of The 12 Principles of Greener Chemistry. This product is aligned with "Waste prevention" and "Designing safer chemicals". Click here for more information.
Application: The GenElute(TM)-E Single Spin Blood DNA 96 Kit has been developed to purify genomic DNA from a wide variety of blood samples, including fresh, frozen or whole blood or dried blood spots (FTA card punches). EDTA-, citrate- as well as heparin-stabilized human and animal blood can be used.The initial blood lysis step takes place under physiological conditions where the enzyme activity of proteases in general is increased. SmartLyse(TM) Protease contains a unique combination of several active enzymes with an expanded substrate specificity. This optimization allows for fast and efficient lysis of blood cells (from, e. g., human, animal, fish, and birds) and dried blood spots (coated FTA cards) with the same protocol.Purified genomic DNA is eluted in Tris buffer, pH 7.8 and can immediately be used for most common downstream applications, including PCR, genotyping, NGS, and others. Final yield is comparable to most common silica-based methods. GenElute(TM)-E purified genomic DNA preparations commonly show an A260/280 ratio of around 1.8.
Legal Information: SmartLyse is a trademark of Merck KGaA, Darmstadt, Germany
Other Notes: We believe that green chemistry will contribute to a better tomorrow. With a growing portfolio of greener alternatives, there are now more choices to reduce the ecological impact of your research while still delivering quality and efficacy so your results are not compromised. GenElute(TM)-E purification kits avoid the use of hazardous wash buffers typically employed in silica-based purification kits, significantly reducing chemical waste. The single-spin protocol also limits the number of collection steps, reducing plastic waste by 55% compared to equivalent bind-wash-elute methods. GenElute(TM)-E is designated a Greener Alternative Product based on the "Prevention" and "Designing Safer Chemicals" qualifications of The 12 Principles of Green Chemistry.GenElute(TM)-E kits also adhere to the principles of SMASH Packaging, our global initiative to improve the sustainability through the reduction of packaging materials, the increased use of sustainable alternatives, and improved recyclability. Packaging is FCS-marked for sustainable forestry practices and produced from greater than 70% recycled content.