GelGreen is a sensitive, stable and environmentally safe green fluorescent nucleic acid dye specifically designed for gel staining. GelGreen has UV absorption between 250 nm and 300 nm and a strong absorption peak centered around 500 nm (Figure 1). Thus, GelGreen is compatible with either a 254 nm UV transilluminator or a gel reader equipped with visible light excitation (such as a 488 nm laser-based gel scanner or a Dark Reader). GelGreen is far more sensitive than SYBR Safe (Figure 2). Unlike SYBR dyes, which are known to be unstable, GelGreen is very stable, both hydrolytically and thermally.The dye is noncytotoxic and non-mutagenic at concentrations well above the working concentrations used in gel staining. GelGreen successfully passed environmental safety tests in compliance with CCR Title 22 Hazardous Waste Characterization, under which GelGreen is not classified as hazardous waste.Spectral PropertiesAbsorbance: Standard Transilluminator (254 nm) such as a Dark ReaderEmission: Long path green filter such as a SYBR filter or GelStar filter.
Synonyms: Fluorescent DNA gel stain
Application: Research Sub CategoryLive Cell Dye
Legal Information: GelGreen is a registered trademark of Biotium Inc.