Application: This animal component-free medium (containing no hypoxanthine or thymidine) is formulated to maximize cell growth and recombinant protein production using the Dihydrofolate Reductase (DHFR) gene amplification system in DHFR- Chinese Hamster Ovary (CHO) cells.
Features and Benefits: Developed to meet the needs of biotechnology and vaccine manufacturing, this medium supports rapid initial cell growth and high levels of protein expression in suspension cultures. This medium supports and maintains high cell densities for extended periods, resulting in increased productivity.
Other Notes: Proprietary formulation containing inorganic salts, HEPES and sodium bicarbonate buffers, essential and non-essential amino acids, vitamins, recombinant human insulin, plant hydrolysates, other organic compounds, trace elements, and surfactants.
Does not contain antibiotics, antimycotics, L-glutamine, or transferrin. Contains no animal-derived proteins or other components.
Caution: Do not freeze. Protect from light. Several components of the medium are light labile and should not be exposed to light for lengthy periods of time.
Legal Information: EX-CELL is a registered trademark of Sigma-Aldrich Co. LLC
Reconstitution: Aseptically add 20-40-áml of 200-ámM L-glutamine solution per liter of medium prior to use
Empirical Formula (Hill Notation): C2H8N2