Application: Complete, chemically-defined medium formulated to optimize cell growth and protein expression in Chinese hamster ovary (CHO) cells.
Features and Benefits: Developed to meet the needs of biotechnology and vaccine manufacturing, this medium supports rapid initial cell growth and high levels of protein expression in suspension cultures. This medium supports and maintains high cell densities for extended periods, resulting in increased productivity.
Other Notes: Proprietary formulation containing inorganic salts, HEPES and sodium bicarbonate buffers, essential and non-essential amino acids, vitamins, recombinant human insulin, other organic compounds, trace elements, and surfactants.
Does not contain antibiotics, antimycotics, L-glutamine, transferrin, hydrolysates, or other undefined nutrients or supplements. Contains no animal-derived components or components synthesized from animal-derived materials.
Caution: Do not freeze. Protect from light. Several components of the medium are light labile and should not be exposed to light for lengthy periods of time.
Legal Information: EX-CELL is a registered trademark of Sigma-Aldrich Co. LLC
Storage: 2-8°C
Reconstitution: Aseptically add 20-40 mL of 200 mM L-glutamine solution per liter of medium prior to use.
Empirical Formula (Hill Notation): C7H4BrF3