Synonym(s): CHO Medium
Application: Animal component-free medium formulated to optimize cell growth and protein expression in Chinese hamster ovary (CHO) cells.
Features and Benefits: Developed to meet the needs of biotechnology and vaccine manufacturing, this medium supports rapid initial cell growth and high levels of protein expression in suspension cultures. It also supports high cell densities and maintenance of these densities of viable cells for extended periods resulting in increased productivity. Cell densities in excess of 8 × 106 cells/ml have been achieved in batch culture systems. Increases in protein productivity up to 500% per cell have been observed.
Other Notes: Proprietary formulation containing inorganic salts, HEPES and sodium bicarbonate buffers, essential and non-essential amino acids, vitamins, recombinant human insulin, plant hydrolysates, other organic compounds, trace elements, and surfactants.
Does not contain antibiotics, antimycotics, L-glutamine, or transferrin. Contains no animal-derived proteins or other components.
Legal Information: EX-CELL is a registered trademark of Sigma-Aldrich Co. LLC
Reconstitution: Aseptically add 20-40 ml of 200 mM L-glutamine solution per liter of medium prior to use.
Empirical Formula (Hill Notation): C6H12N2O