Product Source: rtLIF is expressed in E. coli as a fusion protein with GST using the pGEX expression system, cleaved from GST moiety with thrombin and purified by ion-exchange chromatography.
Synonyms: Rat LIF
Purity: >85% (active component rat LIF (rtLIF), SDS-PAGE)
Application: Applications for Rat ESGRO include use as a reagent for the in vitro maintenance of rat ES cells. Studies have demonstrated that rtLIF is more effective in maintaining the undifferentiated phenotype of rat ES cells than similar concentrations of murine LIF (Takahama et.al. 1998). Rat ES Cells: RtLIF produced from a transfected cell line, has been shown to effectively maintain the undifferentiated phenotype of rat ES cells when used at a concentration of 1000-1500 units / mL (Takahama et.al. 1998). At this recommended concentration 106 units of ESGRO is sufficient for 1L of tissue culture media. Visit www.esgro-lif.com for additional information Rat LIF is not available for resale. For research use only; not for use as a diagnostic.
Legal Information: ESGRO is a registered trademark of Merck KGaA, Darmstadt, Germany