Introduction: Cell adhesion plays a major role in cellular communication and regulation, and is of fundamental importance in the development and maintenance of tissues. Scientists are continually examining the adhesion, migration, and invasion of many diverse cell types on various extracellular matrix (ECM) component proteins. The CHEMICON(R) ECM Cell Adhesion Array Kit (Fluorometric) provides an ECM Array microtiter plate with a homogenous fluorescence detection format, allowing for large-scale screening and quantitative comparison of multiple samples/cell lines. The kit is designed as a rapid (1-2 hours), cost effective, efficient method for characterization of cell adhesion. In addition, Chemicon continues to provide numerous migration, invasion, and adhesion products including: . Alpha Integrin-Mediated Cell Adhesion Array Kit (Colorimetric) (ECM530) . Beta Integrin-Mediated Cell Adhesion Array Kit (Colorimetric) (ECM531) . Alpha/Beta Integrin-Mediated Cell Adhesion Array Combo Kit (Colorimetric) (ECM532) . Alpha Integrin-Mediated Cell Adhesion Array Kit (Fluorometric) (ECM533) . Beta Integrin-Mediated Cell Adhesion Array Kit (Fluorometric) (ECM534) . Alpha/Beta Integrin-Mediated Cell Adhesion Array Combo Kit (Fluorometric) (ECM535) . ECM Cell Adhesion Array Kit (Colorimetric) (ECM540) . QCM 8mum 96-well Chemotaxis Cell Migration Assay (ECM510) . QCM 5mum 96-well Chemotaxis Cell Migration Assay (ECM512) . QCM 3mum 96-well Chemotaxis Cell Migration Assay (ECM515) . QCM 96-well Cell Invasion Assay (ECM555) . QCM 96-well Collagen-Based Cell Invasion Assay (ECM 556) . 24-well Insert Cell Migration and Invasion assay systems . CytoMatrix Cell Adhesion strips (ECM protein coated) . QuantiMatrix ECM protein ELISA kits Test Principle: The CHEMICON ECM Cell Adhesion Array kit utilizes a homogenous fluorescence detection format, allowing large-scale screening and quantitative comparison of multiple samples. Each kit contains a 96-well, microtiter plate consisting of 12 X 8-well removable strips. Each well within a strip (7 wells total) is pre-coated with a different ECM protein (see table below) along with one BSA-coated well (negative control). Experimental cells are seeded onto the coated substrates, where adherent cells are captured. Subsequently, unbound cells are washed away, and the adherent cells are lysed and detected by the patented CyQuant GR dye (Molecular Probes) (1-2). This green-fluorescent dye exhibits strong fluorescence enhancement when bound to cellular nucleic acids (3). Relative cell attachment is determined using a fluorescence plate reader. Additional information about the ECM components provided in the kit:
Application: The ECM Cell Adhesion Array kit is useful for assessing specific cell surface integrins, monitoring in vitro cell differentiation, or screening potential cell adhesion promoters/inhibitors. For Research Use Only. Not for use in diagnostic procedures.
Legal Information: GR is a trademark of Sigma-Aldrich Co. LLC