Ready to use dCas9-VP64 lentiviral particles enable immediate transduction of a wide range of cell lines. dCas9-VP64 lentiviral particles efficiently and stably integrate dCas9-VP64 and blasticidin resistance cassette linked by a 2A peptide and driven by the EF1 alpha promoter (EF1a-dCas9-VP64-2A-Blasticidin) for strong expression in both dividing and non-dividing cell lines. They are ideal for avoiding the tedious lentivirus production process and are one part of a three part CRISPR system with individual dCas9-VP64. MS2-p65-HSF1 and gRNA expression vectors. To order gRNA in any format click here
Storage: -70C
Application: Functional Genomics/Target Validation
•Unbiased forward genetic screening
•Strong transcriptional activation in multiple cell lines
•Creation of cell lines stably expressing dCas9-VP64.
Legal Information: CRISPR Use License AgreementLentiviral, WPRE and Evrogen Fluorophore Licenses