DL-Dithiothreitol, >=98% (HPLC), >=99.0% (titration)

Synonyms: threo-1,4-Dimercapto-2,3-butanediol; Cleland's reagent; DTT

CAS Number: 578517

Formula: C4H10O2S2

Formula Weight: 154.25

Linear Formula: HSCH2CH(OH)CH(OH)CH2SH

MDL No.: MFCD00004877

Purity: >=98% (HPLC); >=99.0% (titration)

Melting Point: 41-44 C (lit.)

Storage: 2-8C

Beilstein Registry Number: 1719757

EC Number: 222-468-7

UNSPSC Code: 12352201

Application: An excellent reagent for maintaining SH groups in reduced state; quantitatively reduces disulfides. DTT is effective in sample buffers for reducing protein disulfide bonds prior to SDS-PAGE. DTT can also be used for reducing the disulfide bridge of the cross-linker N,N′-bis(acryloyl)cystamine to break apart the matrix of a polyacrylamide gel. DTT is less pungent and is less toxic than 2-mercaptoethanol. Typically, a seven fold lower concentration of DTT (100 mM) is needed than is used for 2-mercaptoethanol (5% v/v, 700 mM).

Application: DTT has been used:. as one of the reactants in the reduction and alkylation of αs1-Casein, the major allergen of cow′s milk.. as a component of medium for the demembranation and reactivation of spermatozoa.. to maintain stability of the enzyme as thiol effectively protects the active sites of the biocatalyst.. as a reducing agent to test the specificity of the reaction of N-Ethylmaleimide with sulfhydryl groups.