UNSPSC Code: 12352204
RIDADR: NONH for all modes of transport
During tissue dissociation, parts of the cells are lysed resulting in a release of DNA. Monomolecular DNA may cause clumping of cells. Addition of DNase I to the dissociation buffer leads to a degradation of this extracellular DNA, therby avoiding the loss of cells from undesired clumping.
Bovine pancreatic deoxyribonuclease I (DNase I) is a DNA minor grove-interacting nuclease , which shows relatively low specificity. This protein is composed of two central Î² sheets, each composed of six Î²-strands. This structure is surrounded by extensive loop and Î±-helical regions. This enzyme shares structural similarity to exonuclease III. DNase I is one of the most well characterized endonucleases of mammalian origin.
For life science research only. Not for use in diagnostic procedures.
Activator: Bivalent metal ions (Ca2+, Mg2+)
Working concentration: 0.01 to 1 mg/ml
Note: For each cell type the working concentration has to be determined individually. For optimal activity the enzyme needs 5 mM Mg2+.
Reconstitute the lyophylizate in sterile double-distilled water (10 mg/ml); further dilution with PBS (phosphate buffered saline), HBSS (Hank′s Balanced Salt Solution) or medium.