Storage: −20°C
UNSPSC Code: 41106000
Components: Labeling Mix 5x concentrated; Transcription Buffer 5x concentrated; SP6 RNA Polymerase 20 U/μl; T7 RNA Polymerase 20 U/μl; T3 RNA Polymerase 20 U/μl; Anti-Digoxigenin-AP antibody, Fab fragments 750 U/ml; DNase I, RNase free 10 U/μl; CDP-Star ready-to-use; Actin RNA Probe, DIG-labeled Antisense Probe, length 588 bases 10 ng/μl; DIG Easy Hyb Granules; Blocking Solution 10x concentrated
RIDADR: NONH for all modes of transport
Application:
The DIG Northern Starter Kit produces DIG-labeled RNA probes that can be used in conjunction with the supplied chemiluminescent detection reagents for northern blotting techniques. Using linearized DNA as a template, SP6, T3, or T7 RNA Polymerases are used to incorporate DIG-11-UTP into the RNA transcript. After labeling, the DIG-labeled probe is immediately ready for use in hybridization. For convenience, DIG Easy Hyb can be used for hybridization. The DIG Easy Hyb granules are easily reconstituted by adding 64 ml DEPC-treated (RNase-free) water directly to the bottle (once reconstituted, DIG Easy Hyb is stable for 1 month at room temperature). After hybridization, the hybridization solution containing the DIG-labeled RNA probe can be stored at -15 to -25 °C for future re-use (up to 1 year).
General description:
The DIG Northern Starter Kit was designed for the novice DIG system user. It contains the reagents proven to provide successful, reproducible results in northern blots. Additionally, the more convenient forms of standard DIG system products are included (e.g., CDP Star, ready-to-use). The small number of reactions allows the user to gain a firm foundation using the DIG system, then "graduate" to the standard pack sizes. For optimum success, use this kit with the DIG Wash and Block Buffer Set.The kit is used for the generation of single-stranded, DIG-labeled RNA probes and chemiluminescent detection. Labeled probes are synthesized by the in vitro transcription method.
Other Notes:
For life science research only. Not for use in diagnostic procedures.
Preparation Note:
Working solution: Note: Use sterile, RNase-free solutions and equipment
Assay Time: 9 hours 30 minutes
Sample Materials:
• Linearized plasmid, including the appropriate RNA polymerase promoter sequence (SP6, T3, T7).
• Specially prepared PCR productWorking solution: Note: Use sterile, RNase-free solutions and equipment.
Note: The length of the region to be transcribed should be in the range of 200 to 1,000 bp. To avoid RNase contamination the DNA must be phenolized.