UNSPSC Code: 41106000
Components: DIG-High Prime 5x concentrated; DIG-labeled Control DNA, pBR328 (linearized with Bam HI) 5 μg/ml; DNA Dilution Buffer; Anti-Digoxigenin-AP Conjugate antibody; CSPD ready-to-use; Blocking Solution 10x concentrated; DIG Easy Hyb Granules
RIDADR: NONH for all modes of transport
DIG-High Prime is used for the highly efficient random-primed labeling of DNA probes with DIG-11-dUTP, alkali-labile. DIG-labeled probes are generated at high yield within one hour or after overnight incubation. DIG-High Prime labeled DNA probes are used in a variety of hybridization techniques:
• Southern blots
• Northern blots
• Dot blots
• Colony and plaque hybridizations
• For all types of filter hybridization
• For single-copy gene detection in total genomic DNA, even from organisms with high complexity, for example, human, barley, and wheat
Convenient kit for random-primed labeling of DNA templates with DIG-11-dUTP, alkali-labile, and chemiluminescent detection of the DIG-labeled hybrids. This kit was assembled with convenience in mind, offering ready-to-use CSPD supplied with a dripping device for easy application, ready-made blocking solution, and DIG Easy Hyb granules. The DIG-High Prime mixture includes stabilized Klenow enzyme, nucleotides, primers, and reaction buffer, all in one convenient reagent.
For life science research only. Not for use in diagnostic procedures.
The DIG High Prime DNA Labeling and Detection Starter Kit II uses digoxigenin (DIG), a steroid hapten, to label DNA probes for hybridization and subsequent chemiluminescence detection by enzyme immunoassay. The "random primed" DNA labeling method originally developed by Feinberg and Vogelstein is based on the hybridization of oligonucleotides of all possible sequences to the denatured DNA to be labeled. The input DNA serves solely as a template for the synthesis of labeled DNA, and is not degraded during the reaction, making it possible to label minimal amounts of DNA (10 ng) with this method.The complementary DNA strand is synthesized by Klenow polymerase using the 3′-OH termini of the random oligonucleotides as primers. Modified deoxyribonucleoside triphosphates, labeled with digoxigenin present in the reaction, are incorporated into the newly synthesized complementary DNA strand.