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MilliporeSigma

ROCHE DAPI, 4',6-Diamidine-2'-phenylindole dihydrochloride

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Synonyms: 4′,6-Diamidino-2-phenylindole dihydrochloride; 2-(4-Amidinophenyl)-6-indolecarbamidine dihydrochloride; DAPI dihydrochloride

Empirical Formula (Hill Notation): C16H15N5 ¬? 2HCl

Formula Weight: 350.25

Linear Formula: C16H15N5 ¬? 2HCl

MDL Number: MFCD00012681

Purity: >90%

Beilstein Registry Number: 4894417

UNSPSC Code: 41116100

RIDADR: NONH for all modes of transport

Application:
DAPI is several times more sensitive than ethidium bromide for staining DNA in agarose gels. It may be used for photofootprinting of DNA, to detect annealed probes in blotting applications by specifically visualizing the double-stranded complex, and to study the changes in DNA and analyze DNA content during apoptosis using flow cytometry. DAPI staining has also been shown to be a sensitive and specific detection method for mycoplasma.

Biochem/physiol Actions:
Cell permeable fluorescent minor groove-binding probe for DNA. Binds to the minor groove of double-stranded DNA (preferentially to AT rich DNA), forming a stable complex which fluoresces approximately 20 times greater than DAPI alone.

Other Notes:
For life science research only. Not for use in diagnostic procedures.

Quality:
Purity: >90% (from N)

Preparation Note:
Working solution: Solubility: 25 mg/ml in water


Preparation of stock solution


Dissolve in double-dist. water to a final concentration of 1 to 5 mg/ml.
Note: Do not use any buffers.


Preparation of working solution


Dilute the stock solution with methanol to a final concentration of 1 μg/ml. The working solution is stable at 2 to 8 °C for about 6 months.
Storage conditions (working solution): Stock solution (1 to 5 mg/ml) at -15 to -25 °C for 12 months.
Working solution (1μg/ml) at 2 to 8 °C for about 6 months.

Reconstitution:
In 2 to 10 ml double-dist. water; 1 to 5 mg/ml final concentration.
Note: Prepare aliquots and store at -15 to -25 °C.

Principle:
The fluorescent dye DAPI binds selectively to DNA and forms strongly fluorescent DNA-DAPI complexes with high specificity. DAPI, once added to tissue culture cells, is rapidly taken up into cellular DNA, yielding highly fluorescent nuclei and no detectable cytoplasmic fluorescence. When the cells are contaminated with Mycoplasmas, characteristic discrete fluorescent foci are readily detected over the cytoplasm and sometimes in intercellular spaces.

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Product Detail
Thomas No.
C756W66
Mfr. No.
10236276001
Description
DAPI, pkg of 10 mg

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Product is restricted and can only be purchased by customers with a web profile linked to a Thomas Business Account - Click here to login or create your web profile. If you do not have a Thomas Business Account, please Contact Us and someone will respond with details on how to apply for a business account with us.

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