Synonym: DNase I, Deoxyribonucleate 5'-oligonucleotido-hydrolase
CAS Number: 9003-98-9
EC Number: 232-667-0
Enzyme Commission (EC) Number: 3.1.21.1 ( BRENDA | IUBMB )
MDL number: MFCD00130918
Analysis Note
Protein determined by biuret.
Application
Used for the removal of DNA from protein samples.
Deoxyribonuclease I from bovine pancreas has been used for the identification, localization and expression of two novel human genes similar to deoxyribonuclease I. Deoxyribonuclease I from bovine pancreas has also been used in a study to investigate a new approach to obtaining high-activity RNase, DNase, cholesterolesterase, and trypsin from cattle pancreas.
Preparation Note
Derived from New Zealand-sourced pancreas
Unit Definition
One Kunitz unit will produce a ?A260 of 0.001 per min per mL at pH 5.0 at 25 °C, using DNA, Type I or III as substrate. [Mg2+] = 4.2 mM.
Physical form
Contains calcium chloride