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MilliporeSigma

Deoxyribonuclease I from bovine pancreas

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Synonym: DNase I, Deoxyribonucleate 5'-oligonucleotido-hydrolase

CAS Number: 9003-98-9
EC Number: 232-667-0
Enzyme Commission (EC) Number: 3.1.21.1 ( BRENDA | IUBMB )
MDL number: MFCD00130918

Analysis Note
Protein determined by biuret.

Application
Used for the removal of DNA from protein samples.
Deoxyribonuclease I from bovine pancreas has been used for the identification, localization and expression of two novel human genes similar to deoxyribonuclease I. Deoxyribonuclease I from bovine pancreas has also been used in a study to investigate a new approach to obtaining high-activity RNase, DNase, cholesterolesterase, and trypsin from cattle pancreas.

Preparation Note
Derived from New Zealand-sourced pancreas

Unit Definition
One Kunitz unit will produce a ?A260 of 0.001 per min per mL at pH 5.0 at 25 °C, using DNA, Type I or III as substrate. [Mg2+] = 4.2 mM.

Physical form
Contains calcium chloride

Quality Level PREMIUM
type  Type II
form  lyophilized powder
mol wt  mol wt ~31 kDa
purified by  chromatography
composition  Protein, ≥80%
solubility  0.15 M NaCl: soluble5.0 mg/mL, clear
foreign activity  Chymotrypsin ≤0.01%
  Protease ≤0.005%
  RNase ≤0.01%
storage temp.  −20°C

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Product Detail
Thomas No.
C974H40
Mfr. No.
D4527-10KU
Pkg. Size
10 KU
CAS #
9003-98-9
Description
DEOXYRIBONUCLEASE I TYPE II FROM BOVINE PANCREAS 10KU
list price/quantitytotal
$0.00
Thomas No.
C974H41
Mfr. No.
D4527-20KU
Pkg. Size
20 ku
CAS #
9003-98-9
Description
DEOXYRIBONUCLEASE I TYPE II FROM BOVINE PANCREAS 20KU
list price/quantitytotal
$0.00
Thomas No.
C974H42
Mfr. No.
D4527-40KU
Pkg. Size
40 ku
CAS #
9003-98-9
Description
DEOXYRIBONUCLEASE I TYPE II FROM BOVINE PANCREAS 40KU
list price/quantitytotal
$0.00
Thomas No.
C974H43
Mfr. No.
D4527-200KU
Pkg. Size
200 ku
CAS #
9003-98-9
Description
DEOXYRIBONUCLEASE I TYPE II FROM BOVINE PANCREAS 200KU
list price/quantitytotal
$0.00
Thomas No.
C974H44
Mfr. No.
D4527-500KU
Pkg. Size
500 KU
CAS #
9003-98-9
Description
DEOXYRIBONUCLEASE I TYPE II FROM BOVINE PANCREAS 500KU
list price/quantitytotal
$0.00
$0.00 (0 Items)
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