Synonyms: threo-1,4-Dimercapto-2,3-butanediol; Cleland's reagent; DTT
Formula: HSCH2CH(OH)CH(OH)CH2SH
Formula Weight: 154.25
CAS No.: 3483-12-3
Purity: ≥99.0% (titration)
Storage Temperature: 2-8°C
Application: An excellent reagent for maintaining SH groups in reduced state; quantitatively reduces disulfides. DTT is effective in sample buffers for reducing protein disulfide bonds prior to SDS-PAGE. DTT can also be used for reducing the disulfide bridge of the cross-linker N,N′-bis(acryloyl)cystamine to break apart the matrix of a polyacrylamide gel. DTT is less pungent and is less toxic than 2-mercaptoethanol. Typically, a seven fold lower concentration of DTT (100 mM) is needed than is used for 2-mercaptoethanol (5% v/v, 700 mM).
Abs.: A0.1M/280, H2O ≤0.100
Anion Traces: sulfate (SO42-): ≤0.005%
Assay: ≥99.0% (titration)
Cation Traces: Al: ≤0.0005%
Impurities: ≤0.3% 4,5-dihydroxy-1,2-dithiane
MP: 41-44°C(lit.)
pH: 4.0-6.0 (20-25°C, 0.1 m in H2O)
Product Line: BioXtra
Quality Level: PREMIUM
Solubility: H2O: soluble0.1 M, clear, colorless
Storage Temp.: 2-8°C