Features & Benefits:Reproducible: eliminates aspiration steps that typically lead to diminished/lost sampleConvenient: spin-column format dramatically simplifies sample handlingReversible: simple elution of antigen:antibody complex in either native or denatured formUse: This kit allows for quick and reproducible immunoprecipitation (IP) by using a spin column. The system is more reproducible than regular IP's, which are problematic with regards to washing the protein A/G agarose without disrupting the agarose bed. The binding of the antibody/antigen complex in Catch and Release is reversible, and elution of the immune complex can occur with native or denaturing buffers. The system has been tested successfully with rabbit, mouse, sheep and goat antibodies. IP using human IgG1-4 should be suitable. IP using chicken antibodies or human IgA, IgD, IgE or IgM is not recommended with this kit. Please read the enclosed product manual before use.
Application: Catch and Release v2.0 should not be used with proteins containing a 6-His tag.
Legal Information: UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany