The C2BBe1 cells, a sublcone of Caco-2 cells, correspond to ATCC CRL-2101. The C2BBe1 cells are adenocarcinoma, epithelial cells from a human caucasian male (aged 72 years).The three week production lead time begins on the Monday following a purchase, in the third week the plates are shipped on Tuesday for receipt on Wednesday or Thursday. As a biologic product that is shipped at room temperature the cells must be processed immediately upon receipt. A minimum of 4 assay ready plates is required to purchase. Please allow for 1 week after ordering, for the plates to be seeded. Plates will be shipped to the customer on day 16 of the 21-day culturing process.
Application: The Engineered Control cells can be used for drug permeability assays. They may also be used as the control in conjunction with C2BBe1 Transporter Knockout cell lines for drug/transporter interaction studies. Engineered control cells have been used in the in vitro Caco-2 permeability assay. The following posters and articles demonstrate how Caco-2 cells can be used for cell based assays:Transporter Function in Caco-2 Cells with Targeted P-Glycoprotein, MRP2 and BCRP Gene Knockout Using Zinc Finger NucleasesComparison of Function and Relative Transporter Protein Concentrations in Caco-2 Cells with Single and Double Knockouts of the ABCB1, ABCG2, and ABCC2 GenesCaco-2 Transporter Knockout Cell Based Assays
Legal Information: This product is covered under a consumable purchase agreement for one-time use only. For more information:ADME/Tox Cell Lines License