All-in-one, ready-to-use Cas9 and guide RNA (gRNA) expression plasmids with GUS ReporterCRISPR Plant Cas9 products are intended for Agrobacterium-mediated plant transformation. The products are based on the type IIA CRISPR-Cas9 derived from Streptococcus pyogenes. The native Cas9 coding sequence is codon optimized for expression in monocots and dicots, respectively. The monocot Cas9 constructs contain a monocot U6 promoter for sgRNA expression, and the dicot Cas9 constructs contain a dicot U6 promoter. Arabidopsis seedlings were germinated in 6 well tissue culture plates The seedlings were infected with Agrobacterium which had the CRISPR plasmids with a GUS reporter. After 3-4 days of transfection the GUS expression was detected. b-glucuronidase (GUS) is an enzyme that hydrolyzes colorless glucuronides to yield colored product
Storage: -20C
Application:
•To verify successful integration of T-DNA in plant genome
•GUS receptor wheat gGAPDH control for monocots for Agrobacterium mediated transformation
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