Purity: 98.5% (electrophoresis)
UNSPSC Code: 12352200
RIDADR: NONH for all modes of transport
Features and Benefits:
• Free from fatty acids according to current quality control procedures (suitable for lipid research)
• High quality
• Low content of heavy metal ions
• Long-term stability
Application:
Bovine Serum Albumin, Fraction V, fatty acid free is used
• in lipid research to study lipid metabolism in biological systems
• Identify transport mechanisms in living organisms
• Study ligand binding
• Investigate ionic binding
• In the preparation of solutions during isolation of cardiomyocytes
Biochem/physiol Actions:
The fatty acids that are insoluble in circulating plasma are transported primarily by serum albumin. The human serum albumin and bovine serum albumin share 76% sequence homology. Being the most abundant protein in the circulatory system, it participates in the maintenance of blood pH and carries several endogenous and exogenous compounds, including fatty acids, metal, amino acids, steroids and drugs.
General description:
Highest quality for fatty acid and lipid research.
BSA is a single polypeptide chain consisting of about 583 amino acid residues and no carbohydrates. At pH 5-7, it contains 17 intra-chain disulfide bridges and one sulfhydryl group.
Other Notes:
For life science research only. Not for use in diagnostic procedures.
Quality:
Purity: ≥98.5 % albumin (electrophoresis), 98% protein
Contaminants: <0.2 mg fatty acids/g, triglycerides not detectable (enzymatic), immunoglobulins not detectable (Ouchterlony), ≤5% H2O,≤30 ppm heavy metals
Preparation Note:
Working concentration: 1 to 100 μg/ml
1 to 100 μg/ml
Working solution: Note: To 90 ml of the normal basal medium F 10, M 199 Hanks, RPMI 1640, MEM Dulbecco, MEM Earle, Alpha MEM (containing 10 to 20% fetal bovine serum), add 10 ml BM-Condimed. Do not use as a basal medium or as a replacement for serum. One percent L-glutamine may be added to the mixture.
Note: Do not add antibiotics, as these are already present in BM-Condimed, and additional antibiotics will affect the growth of the cells.
Storage conditions (working solution): In solution, albumin is stable for about three months at -15 to -25 °C.