Storage: 2-8°C
UNSPSC Code: 12352200
Components: Blocking Reagent (green screw cap); Phosphate Buffered Saline (PBS) (blue screw cap); D-Biotinoyl-ε-aminocaproic acid-N-hydroxysuccinimide ester (Biotin-7-NHS); Dimethylsulfoxide (DMSO) (red screw cap); Sephadex G-25 Columns
RIDADR: NONH for all modes of transport
Application:
Biotin protein labeling kit has been used to produce biotinylated denatured bovine serum albumin (d-BSA) for coating cadmium telluride (CdTe) quantum dot (QD) conjugates.
The kit is suitable for the labeling of proteins with biotin for immunochemistry, immunoblotting, and ELISA.
General description:
For labeling of proteins with biotin.
Free amino acids of the protein to be labeled react with D-biotinoyl-ε-aminocaproic acid-N-hydroxy-succinimide ester (biotin-7-NHS) by forming a stable amino bond. Non reacted biotin-7-NHS is separated on a Sephadex G-25 column.
Other Notes:
For life science research only. Not for use in diagnostic procedures.
Quality:
Function tested.
Preparation Note:
Working solution: Preparation of the solutions
• Blocking solution: Dissolve the contents of the bottle blocking reagent (green screw cap) in 300 ml double-distilled water.
• PBS solution: Dissolve the contents of the bottle PBS (blue screw cap) in 1 I double-distilled water.
• Biotin-7-NHS solution: The solution should be freshly prepared before labeling. Add to the contents of the reaction vial which includes biotin-7-NHS 250 μI DMSO and vortex the solution several times. The resulting concentration of the biotin-7-NHS solution is 20 mg/ml. For the labeling mix, the volume of the biotin-7-NHS solution should not exceed 5% of the total reaction volume (e.g., add maximal 50 μl biotin-7-NHS solution to 1 ml protein solution).