Murashige and Skoog Modified Basal Salt Mixture, powder, plant cell culture tested

Synonym: Murashige and Skoog Modified Basal Salt Mixture, w/o Ammonium Nitrate

Formula Variant
Without NH₄NO₃
With the macro- and micronutrients described by Murashige and Skoog (1962).

Preparation Note
Murashige and Skoog medium can be reconstituted from powder or by combining products that are major components of complete M&S medium, such as macronutrient mixtures and vitamin mixtures. Murashige and Skoog Salt mixture (M2909) contain the macronutrients and micronutrients of the original classic formulation without ammonium nitrate. It can be combined with M&S vitamins or Gamborg’s vitamins and supplemented with sucrose, agar, auxins (IAA) and cytokinins (Kinetin) to generate a complete medium for growth plant tissue culture.

Application
Murashige and Skoog medium is a widely used plant tissue culture growth medium. M&S Basal Medium contains macronutrients that include high levels of nitrate and organic additives such as agar, sugars, vitamins and growth regulators. Important growth regulators frequently added to M&S include IAA (auxin/morphogen) and the Kinetin (cytokinin/cell division promoter).