Murashige and Skoog Basal Medium, powder, plant cell culture tested

Synonym: MS Basal Medium

MDL Number: MFCD00240976

Formula Variant
With the macro- and micronutrients, and vitamins as described by Murashige and Skoog (1962).

Preparation Note
Murashige and Skoog medium can be reconstituted from powder or by combining products that are major components of complete M&S medium, such as macronutrient mixtures and vitamin mixtures. Murashige and Skoog medium (M5519) contains the micronutrients and vitamins of the original classic formulation. It can be supplemented with sucrose, agar, auxins (IAA) and cytokinins (Kinetin) to generate a complete medium for growth plant tissue culture.

Quantity
Formulated to contain 4.4 grams of powder per liter of medium.

Application
Murashige and Skoog medium is a widely used plant tissue culture growth medium. M&S Basal Medium contains macronutrients that include high levels of nitrate and organic additives such as agar, sugars, vitamins and growth regulators. Important growth regulators frequently added to M&S include IAA (auxin/morphogen) and the Kinetin (cytokinin/cell division promoter).