Anti-eIF4E antibody produced in rabbit, ~1 mg/mL, affinity isolated antibody, buffered aqueous solution

Synonyms: Anti-CBP; Anti-Eukaryotic intiation factor 4E; Anti-MGC111573; Anti-eIF4E1; Anti-eIF4EL1; Anti-eIF4F

MDL No.: MFCD01633313

Storage: -20C

UNSPSC Code: 12352203

Features and Benefits: Antibody BioguaranteeEvaluate our antibodies with complete peace of mind. If the antibody does not perform in your application, we will issue a full credit or replacement antibody. Learn more.

Application: Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below.Western Blotting (1 paper)

Application: Anti-eIF4E antibody produced in rabbit is suitable for the following applications: . Immunocytochemistry at a concentration of 5-10mug/mL using paraformaldehyde-fixed 3T3 cells . Immunoprecipitation at a concentration of 2-5muL using HeLa cell lysates . Western blotting at a concentration of 0.5-1mug/mL using HeLa cell lysates

Biochem/physiol Actions: Eukaryotic translation initiation factor 4E is a protein encoded by the EIF4E gene in humans. It is located on human chromosome 20. High level of eIF4E is associated with an increased risk for systemic metastasis in node-positive breast cancer. eIF4E over expression is also associated with a higher incidence of metastasis to multiple sites and acts as a marker for molecular events that increases risk for systemic dissemination. It is also involved in maintaining cell proliferation.

Immunogen: synthetic peptide corresponding to amino acids 21-37 of human eIF4E, conjugated to KLH via a C-terminal added cysteine residue. The sequence is conserved in human, rat, and mouse.

Physical form: Solution in 0.01 M phosphate buffered saline, pH 7.4, and 15 mM sodium azide.