Anti-eIF2Bepsilon antibody produced in rabbit, IgG fraction of antiserum, buffered aqueous solution

Synonyms: Anti-Eukaryotic translation initiation factor 2B, epsilon; Anti-Eukaryotic translation initiation factor 2B, subunit 5; Anti-eIF-2B GDP-GTP exchange factor subunit epsilon

Storage: -20C

UNSPSC Code: 12352203

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Application: Anti-eIF2Bepsilon antibody produced in rabbit is suitable for indirect immunofluorescence (at a dilution of 1:500-1:1,000 using paraformaldehyde fixed NIH-3T3 cells) and western blotting at a dilution of 1:500-1:1,000 using Rat1 or HEK-293T cell lysates.Yale Center for High Throughput Cell Biology IF-tested antibodies. Each antibody is tested by immunofluorescence against HUVEC cells using the Yale HTCB IF protocol. To learn more about the Sigma Life Science and Yale Center for High Throughput Cell Biology partnership, visit sigma.com/htcb-if.

Biochem/physiol Actions: Translation initiation factor eIF-2B subunit ε is a protein encoded by the EIF2B5 gene in humans. The eIF2B is composed of five subunits α, β, γ, δ and ε, within which the ε subunit is responsible for catalyzing the guanine exchange reaction. Defect in any of these 5 subunits leads to diseases. Mutation in the subunit of eIF2B is associated with an autosomal recessive leukoencephalopathy called Vanishing white matter (VWM).

Immunogen: synthetic peptide corresponding to amino acids 50-65 of human eIF2epsilon conjugated to KLH. The corresponding sequence is identical in rat and mouse.

Physical form: Solution in 0.01 M phos­phate buffered saline, pH 7.4, containing 15 mM sodium azide.

Target description: eIF2Bepsilon encodes one of five subunits of eukaryotic translation initiation factor 2B (EIF2B), a GTP exchange factor for eukaryotic initiation factor 2 and an essential regulator for protein synthesis.