Synonyms: Anti-CA9 Carbonic anhydrase 9 precursor; Anti-CAIX; Anti-MN
UNSPSC Code: 12352203
Application: Yale Center for High Throughput Cell Biology IF-tested antibodies. Each antibody is tested by immunofluorescence against HUVEC cells using the Yale HTCB IF protocol. To learn more about the Sigma Life Science and Yale Center for High Throughput Cell Biology partnership, visit sigma.com/htcb-if. Anti-CA9 (437-450) antibody produced in rabbit is suitable for western blotting at a working dilution of 1:500-1:2000.
Biochem/physiol Actions: Carbonic anhydrase 9 (CA9/CAIX) is an enzyme encoded by the CA9 gene in humans. It belongs to Carbonic anhydrases (CAs) family that catalyzes the reversible hydration of carbon dioxide. It is a membranously located metalloenzyme that is involved in pH homeostasis with influence on regulation of cell proliferation, oncogenesis and tumor progression. MN/CA IX is the only tumor-associated CA isoenzyme located on chromosome band 9p12-p13. It acts as a marker for renal cell carcinoma. It is used for patients with advanced mesothelioma. CA9 is useful in differentiating CRCC from ChRCC and oncocytomas, distinguishing low-grade CRCC from normal renal tubules in small samples, separating ICC from HCC and identifying metastatic CRCC from other metastases. Under hypoxia the CA9 increases, which is related to poor prognostic factors. It also acts as a functional mediator of cancer progression.
Immunogen: synthetic peptide corresponding to amino acids 437-450 of human CA9
Physical form: Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide
RIDADR: NONH for all modes of transport
WGK Germany: 2