Anti-beta-tubulin antibody, Mouse monoclonal (mouse IgG1 isotype) is derived from the 2-28-33 hybridoma produced by the fusion of mouse myeloma cells and splenocytes from an immunized mouse. Tubulin also known as TUBB is the major building block of microtubules. Microtubules assemble from heterodimers of alpha-tubulin and beta-tubulin; both have a molecular weight of ~ 50 kDa and share considerable homology. For beta-tubulin, five evolutionarily conserved isotypes have been identified, which are regularly utilized in the same cell types of different species.
Application: Monoclonal Anti-ß-tubulin has been used:
Biochem Physiol Actions: Monoclonal Anti-beta-tubulin specifically recognizes the two major beta-tubulin isotypes and one of the minor beta-tubulin isotypes of the free-living soil nematode Caenorhabditis elegans. It also binds to Sarkosyl resistant ribbons from Strongylocentrotus purpuratus sperm axonemes, neuronal axons of the larva of Ciona intestinalis (ascidian), microtubules in root tip cells in Arabidopsis thaliana and to human, mouse, rat, monkey, bovine, dog and chicken beta-tubulin. The different beta-tubulin isotypes are suggested to differ from each other in their ability to polymerize into microtubules.