Synonyms: RPE65
Storage: 2-8C
Application: Western blot: 1:5,000-1:20,000 on bovine RPE membranes using ECL. Suggested dilution buffer is TBS containing 10% calf serum, 0.25% T-20, 1M D-glucose with 10% glycerol. Suggested blocking buffer is TBS containing 2% BSA and 0.5% Tween 20. Preferred gel percentage is 10%. Immunohistochemistry on frozen tissue sections: 1:250-1:500 Immunoprecipitation: 20 mug of antibody in a reaction volume of 500 muL. Immunoaffinity purification ELISA Optimal working dilutions must be determined by end user.
Legal Information: CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
Other Notes: Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.