Prox1, the homolog of Drosophila Prospero, is a homeobox-containing transcription factor that binds and functions as a co-receptor of liver receptor homolog 1 (LRH1/NR5A2). It is a specific marker for lymphatic endothelial cells, as well as hepatoblasts and hepatocytes. Human Prox1 shares 97% amino acid sequence identity with mouse and rat Prox1.
Application: Immunohistochemistry: 1:2,000-1:10,000 with overnight incubation on 4% paraformaldehyde fixed, frozen tissue sections. For embryos immersion fixation is recommended. For older animals perfusion fixation is recommended. Suggested permeabilization solution is 0.1% Triton X100. Suggested blocking buffer is PBS with 0.1% Triton and 10% serum.Immunocytochemistry: A previous lot of this antibody was used on Immunocytochemistry
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Other Notes: Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.