Synonyms: Multidrug Resistance Protein-1
Application: Western Blot: 0.2 mug/mL, with HRP-conjugated anti mouse IgG (Hipfner et al., 1994, 1996). Immunocytochemistry: 1:20 - 1:50 on paraformaldehyde-fixed (0.5% in PBS) or 70% methanol-fixed cytospin preparations (Hipfner et al., 1999). Immunohistochemistry on formalin-fixed paraffini-embedded sections following antigen retrieval by microwave heating in citrate (Wright, et al., 1998). Flow cytometry: 1:20 dilution on cells fixed in 0.5% paraformaldehyde in PBS for 30 minutes, followed by anti-mouse FITC (block and wash with solution containing 0.1% Tween) (Hipfner, et al., 1994). Immunoprecipitation (Hipfner, et al., 1994; 1996). Optimal working dilutions must be determined by the end user.
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